DNA barcoding

DNA barcoding:-

DNA barcoding is a method of species identification using a short section of DNA from a specific gene or genes.

DNA barcoding techniques were developed by Paul D.N. Hebert et al. from

the University of Guelph, Ontario, Canada

Methodology

- Sampling and preservation

o Tissue samples

o Bulk samples

o eDNA samples

- DNA extraction, amplification and sequencing

- Marker selection

Applications:-

Applications of DNA barcoding include-

 Identification of species

 Detection of invasive species

 Delimiting cryptic species

 Diet analysis and food web application

 Barcoding for food safety

 Biomonitoring and ecological assessment

DNA barcoding database-BOLD :-

- The Barcode of Life Data System ( BOLD ) is an informatics workbench aiding the

acquisition, storage, analysis and publication of DNA barcode records.

- By assembling molecular, morphological and distributional data, it bridges a traditional

bioinformatics chasm.

- BOLD is freely available to any researcher with interests in DNA barcoding.

- By providing specialized services, it aids the assembly of records that meet the standards

needed to gain BARCODE designation in the global sequence databases.

- Because of its web-based delivery and flexible data security model, it is also well

positioned to support projects that involve broad research alliances.

- Bold was initially developed as an informatics workbench for a single, high-volume DNA

barcode facility (Hajibabaei et al . 2005).

- It has evolved into a resource for the DNA barcoding community, as evidenced by its

adoption for the first major barcode campaigns (birds, fishes, Lepidoptera).

- It has, as well, been selected by the Canadian Barcode of Life Network (www.bolnet.ca)

for its campaign to barcode all eukaryotic life in that nation.